Biotechnology - Sciene

Many experiments were carried out to investigate the ability of Ruta graveolens to tolerate heavy metals in vitro and to investigate molecular bases of heavy metals tolerance.

       In this study Withania somnifera leaves were collected from gardens of Al-Nahrain University. Then the leaves powder was used to get three type of extracts, each type of Withania extracts (water, ethanol and acetone extract) were subjected to chemical analysis to identify the active compounds in each extract which indicated that alkaloids, glycoside, saponins, flavonoids, fixed oil were present in ethanol, acetone and water extracts, while absent of terpene in water extract and protein in acetone extract, Tannins were not found in all extract types.

This study was designed to evaluate osteogenic potential of mesenchymal stem cells (MSCs) isolated from mouse bone marrow in vitro. MSCs were isolated by collecting the thigh bone of 50 male albino mice, both the femur and tibia were collected and cells were flushed from bones and MSCs isolated based on the ability of adherence to plastic surfaces. Reactivity MSCs to CD105 and CD34 were tested by immunocytochemistry.