Biotechnology - Sciene

   This study was constructed to investigate hyperprolactinemia related infertility through  a biochemical and molecular base   associated with single nucleotide polymorphism (SNP) at prolactin gene and prolactin receptor gene in hyperprolactinemic patients. The study included one hundred fifty blood samples from patients suffering hyperprolactinemia and infertility during the period from March

Many soil samples were collected from different places and screened for PHA production by Sudan Black B stain. Twenty isolates had a positive result for Suddan Black B stain. The results were confirmed by staining the viable colonies with the selective stain Nile Red; three isolates gave positive results. One isolate with a more pink fluorescent with Nile Red stain was selected for the present study.

 This study was designed to shed light on the association between polymorphism of macrophage migration inhibitory factor (MIF) gene at -173 locus as risk factor of bladder cancer in Iraqi population by PCR-RFLP ,estimation the serum level of MIF with some cytokines (IL-6, IL-10 and TNF-α) by sandwich ELISA and  determine the expression of several marker (CD74, CD44, P 53  and P 21 ) by  immunohistochemical staining.