Construction of new Ion Selective electrodes for Determination Chloramphenicol sodium succinate and Iron in pharmaceutical samples

number: 
2659
English
department: 
Degree: 
Author: 
Weam Raad Azeez
Supervisor: 
Assistant professor Dr. Khaleda H. Al-Saidi
year: 
2011

Two kinds of electrodes were prepared in this study based on PVC matrix. Four ion selective electrodes for Chloramphenicol sodium succinate which based on chloramphenicol palmitate.

1. Di-butyl phthalate (DBPH)

2. Di-butyl phosphate (DBP)

3. Di-octyl phthalate (DOP)

4.Tri-butyl phosphate (TBP)

The  electrodes parameters  were  include, linear  concentration  range,Nernestian slope, limit of detection, response time, life time, working pH rang and selectivity were evaluated. Also the statistical treatments were applied for the results that include: relative standard deviation (RSD), relative error (RE), error and confidence limit for concentration. The results showed:1- ISES for Chloramphenicol sodium succinate:CPP+TPB+DBPH (membrane A1), CPP+TPB+DBP(membrane A2), CPP+TPB+DOP (membrane A3), CPP+TPB+TBP (membrane A4), gives the slopes (53.98, 51.45, 49.66 and 48.98 mV/decade), linear range from (1x10-4-1x10-1, 5x10-4-1x10-1, 1x10-4-1x10-1, 5x10-4-1x10-1),  with detection  limit  (5x10-5M, 2x10-5 M, 3x10-5 M and 1x10-5 M), response time of 10-3 M (15, 18, 20 and 35 second) and the lifetime were about (50, 15, 23  and 21 day ).  The  working  pH ranges  were  ranged  from (2–7.5). The  electrode A1  (CPP+TPB+DBPH) has  been used  to  determine Chloramphenicol  sodium  succinate in  the  pharmaceutical samples of (Chloramphenicol sodium succinate injection).2- ISES for Iron(III):CPSS-Fe(III)+DBP  (membrane B1), CPSS-Fe(III)+DBPH (membrane B2), CPSS-Fe(III)+DOP (membrane B3) and  CPSS-Fe(III)+TBP (membrane B4), gives  the  slopes  (19.79, 26.60, 16.01 and 13.82), linear range  from (1x10-5- 1x10-2 M, 1x10-5-1x10-2 M, 1x10-6-1x10-2 M and 1x10-5-1x10-2 M), with  detection  limit  (9×10-6 M, 7×10-5 M, 2×10-6 M and 9×10-5 M), response  time  of 10-3 M (10, 35, 15, 35  and 30 second), lifetime  were  about  (37, 41, 23 and 16 days).  The  working  pH  ranges were  ranged  from (2 - 6) by  using  electrode (CPSS-Fe(III)+DBP),  and this  electrode has been  used  to  determine  Iron(III) in the  pharmaceutical samples of (Feroglobin Capsules).